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Applied Biosystems™ GlobalFiler™ PCR Amplification Kit
Description
The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples and is also available in a format containing an internal quality control (IQC) system (Cat. No. A43565), helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. For database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit (Cat. No. 4476609).
Key Features:
- Efficient protocols minimize amplification time compared to previous-generation kits
- An optimized buffer system and expanded DNA input volume delivers maximum sensitivity for trace/low level DNA samples
- Enhanced intracolor balance simplifies interpretation of mixture samples
- The inclusion of 10 mini-STRs maximizes results from degraded samples
- The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility
The GlobalFiler kit is a robust and reliable single-amplification STR multiplex kit optimized for use with the most challenging casework samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases, including 10 mini-STRs, designed to deliver optimized results with even highly degraded samples.
Developed specifically for casework applications, the GlobalFiler kit contains a buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples. The GlobalFiler kit utilizes the latest advances in oligo synthesis and purification, which provide a clean baseline and a balanced profile, resulting in simplified data analysis. Maintenance of the NGM SElect and Identifiler primer sequences helps ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).
Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
Specifications
Specifications
| Content And Storage | Store at -15°C to -25°C upon receipt and 2 to 8°C after initial use for up to the expiration date stated on the kit. |
| Detection Method | Laser-induced Fluorescence |
| Format | Tube |
| Sample Type | DNA (Genomic), Forensic Sample |
| For Use With (Equipment) | Veriti Pro Thermal Cycler, ProFlex Thermal Cycler, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, SeqStudio Genetic Analyzer, SeqStudio 8 Flex Genetic Analyzer, SeqStudio 24 Flex Genetic Analyzer |
| GC-Rich PCR Performance | High |
| For Use With (Application) | Real Time PCR (qPCR) |
| Label or Dye | FAM, VIC, NED, TAZ, SID, LIZ |
| Matrix and Size Standards | GeneScan 600 LIZ Size Standard v2.0, Matrix Standard Set DS-36 (Dye Set J6) |
| No. of Reactions | 1000 Reactions |
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