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Biotium GloMelt™ Thermal Shift Protein Stability Kit
Supplier: Biotium 330211
GloMelt™ dye undergoes fluorescence enhancement upon binding to hydrophobic regions of denatured proteins. Therefore the dye can be used to detect protein unfolding or measure thermal stability by performing a thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. GloMelt™ dye has significant advantages over other environmentally sensitive dyes, such as SYPRO Orange and PROTEOSTAT TS dye. GloMelt™ dye generates a strong signal because it is optimized for detection in the SYBR Green channel of qPCR instruments, and therefore low reaction volumes and low protein concentrations can be used. GloMelt™ dye is compatible with high concentrations of protein stabilizers (such as glycerol and sorbitol), and also protein destabilizers. GloMelt™ dye performs very well in high detergent concentrations, unlike SYPRO Orange. This kit contains no ROX reference dye and has sufficient reagent for 2000 assays.
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