Invitrogen™ Human GLP-1 7-36a ELISA Kit

Catalog No.	Quantity
EHGLPX5	5 x 96 Tests
EHGLP	96 Tests

Catalog No. EHGLPX5 Supplier Invitrogen™ Supplier No. EHGLPX5

Description

ELISA

The Human GLP-1 7-36a ELISA quantitates Hu GLP-1 7-36a in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GLP-1 7-36a. Principle of the method The Human GLP-1 7-36a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

GCG is also known as GLP1, or Glucagon. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis.It is mapped to 2q36-2q37. GLP1, also known as 7-37 for the codons of the preproglucagon molecule which encode it, renders pancreatic beta-cells 'glucose-competent' and may be useful in the treatment of noninsulin-dependent diabetes mellitus. Also, GLP1 is a potent insulin secretagogue.It plays a major role in the enteroinsular axis, accounting, for example, for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously.

Specifications

• Accession Number: P01275
• Assay Range: 156.3 to 20,000 pg/mL
• Assay Sensitivity: 150 pg/mL
• Calibration Range: 150 pg/mL to 20,000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: GCPR
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects human GLP-1 (7-36 A). Other species not determined
• Sufficient For: 5 x 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 2641
• Gene Symbol: GLP1,GLP2,GRPP
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Signal Transduction
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: GLP1, GLP2, GRPP
• Target: GLP-1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins