Invitrogen™ Human GLP-1 ELISA Kit

Catalog No.	Quantity
50-180-40	96 Tests

Catalog No. 50-180-40 Supplier Invitrogen™ Supplier No. BMS2194

Description

ELISA

The Glucagon Like Peptide-1 (GLP-1) ELISA quantitates GLP-1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant GLP-1 in Human samples. Principle of the method The GLP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

GCG is also known as GLP1, or Glucagon. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis.It is mapped to 2q36-2q37. GLP1, also known as 7-37 for the codons of the preproglucagon molecule which encode it, renders pancreatic beta-cells 'glucose-competent' and may be useful in the treatment of noninsulin-dependent diabetes mellitus. Also, GLP1 is a potent insulin secretagogue.It plays a major role in the enteroinsular axis, accounting, for example, for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously.

Specifications

• Accession Number: P01275
• Assay Range: 0.8-50.0 pg/mL
• Assay Sensitivity: 0.32 pg/mL
• Content And Storage: Aluminum pouch(es) with a Microplate coated with monoclonal antibody to GLP-1
  Biotin-Conjugate anti-GLP-1 monoclonal antibody
  Streptavidin-HRP
  GLP-1 Standard lyophilized, 100 pg/mL upon reconstitution
  Sample Diluent
  Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  Substrate Solution (tetramethyl-benzidine)
  Stop Solution (1M Phosphoric acid)
  Adhesive Films
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: GCG; Glicentin; Glicentin-related polypeptide; GLP1; GLP-1; GLP-1(7-36); GLP-1(7-37); GLP1/2; GLP2; GLP-2; Glu; glucagon; Glucagon like peptide 1; Glucagon-like peptide 1; Glucagon-like peptide 1(7-36); Glucagon-like peptide 1(7-37); Glucagon-like peptide 2; glucagon-like peptide-1; GRPP; Incretin hormone; MGC138331; OXM; OXY; Oxyntomodulin; PPG; preproglucagonGCG; Glicentin; Glicentin-related polypeptide; GLP1; GLP-1; GLP-1(7-36); GLP-1(7-37); GLP1/2; GLP2; GLP-2; Glu; glucagon; Glucagon like peptide 1; Glucagon-like peptide 1; Glucagon-like peptide 1(7-36); Glucagon-like peptide 1(7-37); Glucagon-like peptide 2; glucagon-like peptide-1; GRPP; Incretin hormone; MGC138331; OXM; OXY; Oxyntomodulin; PPG; preproglucagon; GLP1; GLP2; GRPP
• Gene ID (Entrez): 2641
• Gene Symbol: GCG
• Interassay CV: 3.8%
• Intraassay CV: 3.8%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, SAV-HRP, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: GRPP, OXM, OXY, GLP-1, Incretin hormone, GLP-1(7-37), GLP-1(7-36), GLP-2
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Amniotic Fluid, 10 μL; Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL
• Storage Requirements: 2-8°C
• Target: GLP-1
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min