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Innovative Research Inc Glucocorticoid Kit
Supplier: Innovative Research Inc IMLGCKT
Glucocorticoid ELISA Kit - This kit is for the quantitative determination of Glucocorticoid in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a competitive inhibition ELISA in strip well format with reagents for up to 96 tests . Glucocorticoid present in samples reacts with an anti-Glucocorticoid monoclonal capture antibody or antigen coated and dried on a microtiter plate. Then, Glucocorticoid conjugated to biotin competes with the antigen in the sample to bind the capture antibody or antigen. Unbound conjugate and sample antigen is washed away and bound conjugate is then reacted with avidin conjugated to HRP. TMB substrate is used for color development at 450nm. Color development is inverse proportional to the concentration of active Glucocorticoid in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Glucocorticoid. For RUO.
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