Invitrogen™ Human Glucocorticoid Receptor Recombinant Protein

Catalog No.	Quantity
A15663	8.3 mg

Catalog No. A15663 Supplier Invitrogen™ Supplier No. A15663

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Glucocorticoid receptor recombinant protein is an insect cell-expressed, full-length, untagged, human nuclear hormone receptor that is partially purified in order to maintain high ligand binding activity. Further purification results in decreased activity, presumably due to loss of endogenous accessory proteins. GR human recombinant protein is: Flexible-use in your in-house assay or as part of our PolarScreen™ Glucocorticoid Receptor Competitor assays for a complete assay solution Ready to use-active and native (untagged) Convenient-available in bulk sizes Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond. Used in:PolarScreen™ Glucocorticoid Receptor Competitor Assay, RedPolarScreen™ Glucocorticoid Receptor Competitor Assay, Green Protein Form: Recombinant, Full Length.

Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity.

Specifications

• Accession Number: P04150
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: Proprietary buffer with no preservative
• Gene ID (Entrez): 2908
• Molecular Weight (g/mol): 85.7 kDa
• Name: Human Glucocorticoid Receptor
• Purification Method: Purified
• Quantity: 8.3 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: cytosolic/nuclear receptor; fb13f09; GCCR; Gcr; GCRST; glucocorticoid nuclear receptor variant 1; glucocorticoid receptor; glucocorticoid receptor 1; glucocorticoid receptor alpha; GR; gr alpha; GR Kit; Grl; Grl1; Grl-1; Nr3c1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858; utouto; utut; wu:fb13f09; zgc:113038
• Common Name: Glucocorticoid Receptor (Nr3c1)
• Gene Symbol: Nr3c1
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Expression System: Baculovirus
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Full Length
• Form: Liquid
• Purity or Quality Grade: Partially purified to retain activity.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov