Cell Biolabs Inc Glycogen Assay Kit (Fluorometric), 100 assays

Supplier:  Cell Biolabs Inc MET5023

Cell Biolabs’ Glycogen Assay Kit (Fluorometric) is a simple assay that measures the amount of total glycogen present in biological samples in a 96-well fluorescence plate reader. Glycogen is broken down into glucose monomers by amyloglucosidase first, glucose is then oxidized by glucose oxidase into D-gluconic acid and hydrogen peroxide. The resulting hydrogen peroxide is then detected with a highly specific fluorometric probe in a standard 96-well colorimetric plate reader. Sample glycogen concentrations are determined by comparison with a known glycogen standard. The kit has a detection sensitivity limit of 120 nM glycogen. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, glycogen standards, and unknown samples. Note each sample replicate requires 2 assays, one of which is a negative control to be subtracted as background.

Catalog No. 50-109-8841