Glycohyodeoxycholic acid (CAS No 13042-33-6) is a bile acid derivative prominent in biomedical research particularly in the study of liver diseases and metabolic disorders Originating from the primary bile acids it plays a crucial role in modulating nuclear receptor pathways such as FXR and TGR5 which are pivotal in regulating bile acid homeostasis and glucose metabolism Its in vitro activity is generally observed in the nanomolar to low micromolar range indicating significant potency Typical applications include usage in various cell models for studying cholesterol metabolism and evaluating hepatoprotective properties as well as in animal studies assessing therapeutic efficacy in liver and metabolic disease models This compound is also explored in drug screening processes aimed at developing treatments for cholestatic liver diseases Experimental concentrations typically depend on the specific research design ensuring tailored and precise analytical results