Thermo Scientific™ GlycoLink™ Micro Immobilization Kit

Description

Immobilize polyclonal antibodies or other glycoproteins, via periodate-oxidized sugars, onto hydrazide-activated polyacrylamide beads for AP, IP or pull-down assays.

Thermo Scientific™ GlycoLink Immobilization Kits provide affinity columns and reagents to immobilize glycoproteins through oxidized sugar groups for use in affinity-purification applications or studies of protein-protein interactions.

The GlycoLink Kits use hydrazide-activated resin and a special catalyst to prepare affinity columns with polyclonal antibodies, which typically have abundant carbohydrates (glycosylation) on their Fc portions. Any glycosylated proteins (even monoclonal antibodies) that contain periodate-oxidizable carbohydrate groups can be immobilized with these kits to make reusable affinity columns. Antibodies immobilized by this hydrazide immobilization chemistry have unobstructed antigen-binding sites and optimal purification capability. Prepared columns with stable glycoproteins or antibodies can be regenerated and reused at least five times for affinity-purification without significant loss in binding capacity.

Highlights:

• High-capacity – immobilize 1 to 10mg of oxidized antibody or other glycoprotein per milliliter of resin

• Fast and efficient – immobilize in as few as 30 minutes; achieve at least 90% coupling of most glycoproteins in less than 4 hours (efficiency depends on the amount and type of glycosylation)

• Specific immobilization – the hydrazide-activated UltraLink Resin conjugates only to purified glycoproteins whose sugar (e.g., sialic acid) groups have been gently oxidized with periodate

• Stable attachment – resonance structure of the hydrazone bonds are sufficiently stable for multiple affinity purifications with one batch of prepared resin; no stabilization step is required

• Preserves antibody function – immobilizes IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target

• Convenient sizes – choose from two complete 10-column kits: one with 1mL columns and one with 0.1mL columns

UltraLink Hydrazide Resin:

• Ligand: hydrazide
• Support: Thermo Scientific UltraLink Biosupport
• Spacer arm: 22 atoms
• Loading: activated with at least 15μmol hydrazide per milliliter of settled resin
• Format: 50% resin slurry in 0.02% sodium azide

Includes:

Kits contain GlycoLink Columns; coupling buffer, coupling catalyst and wash buffer; oxidizing reagent; desalting columns, PBS buffer pack; and column accessories

Recommended for:

Purify antigens using covalently coupled antibodies; Determine binding partners for a glycoprotein of interest; Perform large-scale affinity purifications

Specifications

• Capacity (Metric): Immobilizing 50 to 500 μg of GlycoProtein per Column
• Description: GlycoLink Micro Immobilization Kit
• Content And Storage: Sufficient For: Immobilizing 50 to 500 μg of glycoprotein per column
  • GlycoLink Columns, 0.1 mL, 10 columns
  • GlycoLink Coupling Buffer, 100 mL
  • Aniline, 0.2 mL
  • Wash Buffer, 60 mL
  • Sodium meta-periodate, 0.5 g
  • Zeba Spin Desalting Columns, 0.5 mL, 10 columns
  • PBS Pack (makes 500mL), 1 pack
  • Column Accessories, 10 ea
  
  Store at 4°C.
• Product Line: GlycoLink™
• Quantity: 10 Columns
• Type: Immobilization Kit
• Stationary Phase: Hydrazide
• Sufficient For: 10 Columns
• Format: Kit, Microcentrifuge, Spin Column
• Column Type: Affinity, UltraLink™ Resin

For Research Use Only. Not for use in diagnostic procedures.