Recombinant Protein;25UG;FITC-Labeled Human Glypican 3, His Tag (Cat. No. GP3-HF2H1) is expressed from human HEK293 cells. It contains AA Gln 25 - His 559 (Accession # P51654-1). It is the FITC labeled form of Human Glypican 3, His Tag (Cat. No. GP3-H52H4).;This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 24.6 kDa respectively. The protein migrates as 40 kDa, 32 kDa and 66-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Anti-hGPC3 antibody (Human IgG1) at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Glypican 3, His Tag (Cat. No. GP3-HF2H1) with a linear range of 0.078-0.625 μg/mL (QC tested).;293 cells were transfected with anti-GPC3-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human Glypican 3, His Tag (Cat. No. GP3-HF2H1, 3 µg/ml) and C. F