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Gibco™ GM-CSF Recombinant Human Protein
Description
- Involved in many biological responses including growth and development of granulocyte and macrophage progenitor cells
- For stimulation and initiation of differentiation of myeloblasts and monoblasts, and chemotaxis of eosinophils
- ED50 range = 0.02 to 0.1 ng/mL, determined by dose dependent proliferation of human TF-1 cells
- Optimal concentration for individual application should be determined by dose response assay
Order Info
Shipping Condition: Wet ice
Specifications
Specifications
| For Use With (Application) | Cell Culture |
| Molecular Weight (g/mol) | 14 kDa |
| Purification Method | Gel-Purified |
| Quantity | 1 mg |
| Source | Human |
| Gene Alias | GM-CSF |
| Product Type | Recombinant Protein |
| Species | Human |
| Recombinant | Recombinant |
| Content And Storage | Lyophilized protein can be stored at 2-8°C dessicated. Once reconstituted, store in aliquots at ≤20°C. Avoid multiple freeze and thaw cycles. |
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Frequently Asked Questions (FAQs)
Gibco recombinant proteins are frequently formulated without carrier proteins or additives (e.g., BSA, HSA, sucrose, etc.) and no Gibco PeproTech recombinant protein contains a carrier protein. As a result, during lyophilization, the protein product may be deposited on the vial as a thin, and sometimes invisible, film instead of a pellet. The size of the pellet, if any, is not directly related to the quantity of the recombinant protein in the vial. Our quality control procedures assure that each vial contains the correct amount of product.
To ensure complete recovery of protein product, before opening a vial of lyophilized recombinant protein, we recommend centrifuging it in a microcentrifuge for 20-30 seconds to drive any protein that may be lodged in the cap or on the side to the bottom of the vial. After reconstitution, you can confirm the presence of product protein by running a small amount on SDS-PAGE. In general, a protein band with expected size should be visible with as little as 10 ng of protein loaded on an acrylamide gel.
Assay time is critical. Each assay needs to beoptimized and performed at the peak response time. Different cells may respond differently to a growth factor or cytokine. We suggest repeating our QC assay using same indicator cells as suggested in the manual to see if you can obtain a similar response. In addition, serum may be masking the response. Serum starvation might be needed for certain types of assays.
Protein solutions are generally not very stable when frozen at low concentration. Upon freeze and thaw, some proteins in the solution may stick to the wall of the container, which results in significant reduction of protein concentration if the starting concentration was low. Therefore, carrier proteins are used to reduce such loss. The most commonly used carrier proteins include bovine serum albumin (BSA), human serum albumin (HSA), or fetal bovine serum (FBS). These carrier proteins are generally used at 0.1% concentration. As a rule of thumb, if the concentration of the recombinant protein is less than 0.5 mg/mL, it is a good idea to add some carrier protein
Lyophilized proteins can typically be stored at 2 to 8 degrees C for several weeks, or stored dessicated at -20 degrees C for long-term storage.
For Research Use Only. Not for use in diagnostic procedures.
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