Invitrogen™ Goat anti-Human IgG F(ab')2 Secondary Antibody, AP

Goat Polyclonal Secondary Antibody

Supplier:  Invitrogen™ 31312

Catalog No. PI31312

Description

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31312 has been successfully used in Western blot applications. Product # 31312 reacts with the light chains of human IgG and with the light chains common to other human immunoglobulins, but not with the Fc portion of human IgG or non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Reconstitute with 1.0 mL of distilled water (0.6 mg/mL after restoration). Country of Origin: USA.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Human IgG F(ab')2
• Classification: Polyclonal
• Conjugate: Alkaline Phosphatase (AP)
• Host Species: Goat
• Quantity: 1 mL
• Primary or Secondary: Secondary
• Content And Storage: 4°C
• Form: Lyophilized

• Applications: Western Blot
• Concentration: 0.6 mg/mL
• Formulation: TBS with 15mg/mL BSA and 0.05% sodium azide; pH 8
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Human
• Product Type: Secondary Antibody
• Isotype: IgG