Invitrogen™ Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody

Goat Polyclonal Secondary Antibody

Supplier:  Invitrogen™ 31125

Catalog No. PI31125

Description

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31125 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31125 reacts with the heavy chains of human IgG from only the Fc portion, but not with the light chains or the Fab fragment common to most human immunoglobulins. This antibody does not react against human IgM, IgA or non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product at 4°C until opened. After opening, dilute only enough antibody for a single day's use. Store remainder at 4°C under sterile conditions. Country of origin: USA.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Human IgG Fc Cross-Adsorbed
• Classification: Polyclonal
• Conjugate: Unconjugated
• Host Species: Goat
• Quantity: 1 mg
• Primary or Secondary: Secondary
• Content And Storage: 4°C
• Form: Liquid
• Green Features: Responsibly packaged

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 1.3 mg/mL
• Formulation: PBS with no preservative; pH 7.6
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Human
• Product Type: Secondary Antibody
• Isotype: IgG

Safety and Handling

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