Invitrogen™ Goat anti-Human IgG (H+L) Secondary Antibody, Biotin

Goat Polyclonal Secondary Antibody

Supplier:  Invitrogen™ 31770

Catalog No. PI31770

Description

Product # 31770 has been successfully used in Western blot, IF, ICC, IHC and ISH applications. Product # 31770 reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins, but does not react against non-immunoglobulin serum proteins. Based on solid-phase enzyme immunoassay, this antibody cross-reacts with horse IgG, human IgM, swine IgG (6%), cat IgG, chicken IgG, dog IgG, dog IgM, guinea pig IgG, mouse IgM, swine IgM (3%), mouse IgG, rabbit IgG, rat IgG, rat IgM (1%), and it has minimal cross-reactivity with immunoglobulins from other species.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Human IgG (H+L)
• Classification: Polyclonal
• Conjugate: Biotin
• Host Species: Goat
• Quantity: 1.5 mg
• Primary or Secondary: Secondary
• Content And Storage: 4°C
• Form: Liquid

• Applications: In Situ Hybridization (ISH), Immunocytochemistry, Immunohistochemistry, Western Blot, ELISA
• Concentration: 1.5 mg/mL
• Formulation: PBS with 3mg/mL BSA and 0.08% sodium azide; pH 7.8
• Purification Method: purified
• Regulatory Status: RUO
• Target Species: Human
• Product Type: Secondary Antibody
• Isotype: IgG

Safety and Handling

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