Invitrogen™ Goat anti-Human IgG (H+L) Superclonal™ Secondary Antibody, PE

Catalog No.	Quantity
A56015PE	500 μL

Catalog No. A56015PE Supplier Invitrogen™ Supplier No. A56015PE

Description

Goat Recombinant Superclonal Secondary Antibody

Goat anti-Human IgG (H+L) Superclonal™ Recombinant Secondary Antibody, PE is specific to Human IgG with minimum cross-reactivity towards Guinea pig IgG, Rat IgG, Horse IgG and Monkey IgM. Moderate cross-reactivity was observed with Monkey IgG. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity, but not photostability. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Human IgG (H+L)
• Applications: Flow Cytometry
• Classification: Recombinant Superclonal
• Concentration: 0.2 mg/mL
• Conjugate: PE
• Formulation: PBS with 5mM sodium azide; pH 7.4
• Host Species: Goat
• Immunogen: Purified Human IgG.
• Purification Method: Affinity chromatography
• Quantity: 500 μL
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: IgG