Invitrogen™ F(ab)-Goat anti-Human IgG (H+L) Superclonal™ Secondary Antibody

Catalog No.	Quantity
SA510375	250 μL

Catalog No. SA510375 Supplier Invitrogen™ Supplier No. SA510375

Description

F(ab)-Goat Recombinant Superclonal Secondary Antibody

F(ab)-Goat anti-Human IgG (H+L), Superclonal™ Recombinant Secondary Antibody specifically detects human IgG Heavy and the Light chain with moderate cross reactivity to Rat IgG and Monkey IgG. No cross-reactivity was observed towards other species of immunoglobulins tested. Smaller F(ab) antibody fragments offer several benefits over whole IgGs, due to their smaller size (~50 kD vs. ~150 kD) and the absence of the Fc region. The smaller size is expected to allow conjugated F(ab) fragments to better penetration into tissue sections and improved staining in applications such as IHC. The lack of Fc region results in reduced nonspecific background staining when staining cells/ tissues with Fc receptors (such as immune cells) and other Fc interaction-related interference. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Human IgG (H+L)
• Applications: ELISA, Western Blot, Immunocytochemistry
• Classification: Recombinant Superclonal
• Concentration: 1.0 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Host Species: Goat
• Immunogen: Purified Human IgG.
• Purification Method: Affinity chromatography
• Quantity: 250 μL
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: IgG F(ab)