Invitrogen™ Goat anti-Mouse IgG F(ab')2 Secondary Antibody, Biotin

Goat Polyclonal Secondary Antibody

Supplier:  Invitrogen™ 31803

Catalog No. PI31803

Description

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31803 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31803 reacts with the light chains of mouse IgG and with those common to other mouse immunoglobulins. This antibody does not react with the Fc portion of mouse IgG or non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Rehydrate with 2 mL distilled water and centrifuge if not clear. Country of Origin: USA.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG F(ab')2
• Classification: Polyclonal
• Conjugate: Biotin
• Host Species: Goat
• Quantity: 2 mL
• Primary or Secondary: Secondary
• Content And Storage: 4°C
• Form: Lyophilized

• Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 1.5 mg/mL
• Formulation: PBS with 15mg/mL BSA and 0.05% sodium azide; pH 7.6
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Product Type: Secondary Antibody
• Isotype: IgG

Safety and Handling

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