Learn More
Goat Anti-Mouse IgG (H&L) Antibody (Alkaline Phosphatase), ProSci

Description
Specifications
Specifications
| Antigen | Goat Anti-Mouse IgG (H&L) |
| Applications | ELISA, Western Blot, Immunohistochemistry |
| Classification | Polyclonal |
| Concentration | 1.0 mg/mL |
| Conjugate | Alkaline Phosphatase (AP) |
| Dilution | Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:2,000 - 1:8,000, Western Blot 1:500 - 1:4,000, Immunohistochemistry 1:500 - 1:2,000. (Optimal dilution should be determined by user). |
| Formulation | 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.1% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (BSA) |
| Host Species | Goat |
| Immunogen | Mouse IgG - whole molecule |
| Purification Method | Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins. |
| Show More |
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.