Invitrogen™ Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, AP

Catalog No.	Quantity
G21060	1 mg

Catalog No. G21060 Supplier Invitrogen™ Supplier No. G21060

Description

Goat Polyclonal Secondary Antibody

The specific activity of the AP conjugates is approximately 150-350 units/mg. One unit is defined as the amount of enzymatic activity required to hydrolyze 1.0 micromole of p nitrophenyl phosphate to p-nitrophenol and inorganic phosphate in 60 seconds at 21°C, pH 10. Reconstituted using 0.5 mL of deionized water to make a 2 mg/mL stock solution.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG (H+L) Cross-Adsorbed
• Applications: ELISA, Immunohistochemistry, Western Blot
• Classification: Polyclonal
• Concentration: 2 mg/mL
• Conjugate: Alkaline Phosphatase (AP)
• Formulation: 50mM tris with 30mM trehalose, 0.1M NaCl and no preservative; pH 8.0
• Host Species: Goat
• Immunogen: Gamma Immunoglobins Heavy and Light chains.
• Purification Method: Purified
• Quantity: 1 mg
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Mouse
• Content And Storage: -20°C
• Product Type: Secondary Antibody
• Form: Lyophilized
• Isotype: IgG