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Invitrogen™ Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, APC

Catalog No. A865
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A865 500 μL
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Catalog No. A865 Supplier Invitrogen™ Supplier No. A865
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Description

Goat Polyclonal Secondary Antibody

The allophycocyanin (APC), crosslinked, goat anti-mouse IgG is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. Because this phycobiliprotein is optimally excited at 633 nm but can also be excited at 594 nm, it can be used with samples simultaneously labeled with Texas Red dye or Alexa Fluor 594 dye without a second excitation source. Fluorescence of this long-wavelength Alexa Fluor dye is not visible by looking through a conventional fluorescence microscope. APC can be used in flow cytometry and imaging applications and is more photostable than Cy5 conjugates.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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Specifications

Antigen Mouse IgG (H+L) Cross-Adsorbed
Applications Flow Cytometry, Immunohistochemistry, Immunocytochemistry
Classification Polyclonal
Concentration 1 mg/mL
Conjugate APC
Formulation PBS with 5mM sodium azide; pH 7.5
Host Species Goat
Immunogen Gamma Immunoglobins Heavy and Light chains.
Purification Method Purified
Quantity 500 μL
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Mouse
Content And Storage 4°C, store in dark
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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