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Invitrogen™ Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, DSB-X Biotin

Catalog No. D20691
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D20691 1 mg
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Catalog No. D20691 Supplier Invitrogen™ Supplier No. D20691
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Description

Goat Polyclonal Secondary Antibody

DSB-X biotin is a derivative of desthiobiotin, a stable biotin percursuor. DSB-X biotin utilizes a seven-atom spacer to increase the available of the DSB-X biotin conjugate to bind in the deep biotin-binding pocket of streptpavidin or avidin. Whereas harsh chaotropic agents and low pH are required to dissociate a biotin complex from avidin or steptavidin, binding can be reversed by adding excess D-desthiobiotin or natural D-biotin at neutral pH and at room temperature.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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Specifications

Antigen Mouse IgG (H+L) Highly Cross-Adsorbed
Applications Immunocytochemistry
Classification Polyclonal
Concentration 2 mg/mL
Conjugate DSB-X Biotin
Formulation PBS with 5mM sodium azide; pH 7.5
Host Species Goat
Immunogen Gamma Immunoglobins Heavy and Light chains.
Purification Method Purified
Quantity 1 mg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Mouse
Content And Storage 4°C, store in dark
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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