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Invitrogen™ Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight™ 350

Catalog No. PI62271
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PI62271 1 mL
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Catalog No. PI62271 Supplier Invitrogen™ Supplier No. 62271
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Description

Goat Polyclonal Secondary Antibody

Product # 62271 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 62271 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 350 Amax= 353 nm; Emax= 432 nm. Mole Dye/Mole Protein Ratio is lot-dependent.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Mouse IgG (H+L)
Applications Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry
Classification Polyclonal
Concentration 1 mg/mL
Conjugate DyLight 350
Formulation PBS with 1% BSA and 0.02% sodium azide; pH 7.2
Host Species Goat
Immunogen Purified Mouse IgG, whole molecule.
Purification Method Antigen affinity chromatography
Quantity 1 mL
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Mouse
Content And Storage 4°C
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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Safety and Handling

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