Invitrogen™ Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight™ 350

Goat Polyclonal Secondary Antibody

Supplier:  Invitrogen™ 62271

Catalog No. PI62271

Description

Product # 62271 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 62271 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 350 Amax= 353 nm; Emax= 432 nm. Mole Dye/Mole Protein Ratio is lot-dependent.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG (H+L)
• Classification: Polyclonal
• Conjugate: DyLight 350
• Host Species: Goat
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Product Type: Secondary Antibody
• Isotype: IgG

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA and 0.02% sodium azide; pH 7.2
• Immunogen: Purified Mouse IgG, whole molecule.
• Quantity: 1 mL
• Primary or Secondary: Secondary
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

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