Invitrogen™ Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody

Goat Polyclonal Secondary Antibody

Supplier:  Invitrogen™ 31236

Catalog No. PI31236

Description

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31236 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Prodcut # 31236 reacts with the heavy chains of mouse IgG1, but not with the heavy chains of other mouse IgG subclasses or with light chains of most mouse immunoglobulins. This antibody does not react against mouse IgM, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and rabbit serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Country of Origin: USA.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG1 Cross-Adsorbed
• Classification: Polyclonal
• Conjugate: Unconjugated
• Host Species: Goat
• Quantity: 1 mg
• Primary or Secondary: Secondary
• Content And Storage: 4°C
• Form: Liquid

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 1.3 mg/mL
• Formulation: PBS with no preservative; pH 7.6
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Product Type: Secondary Antibody
• Isotype: IgG