Invitrogen™ Goat anti-Mouse IgG1, IgG2a, IgG2b, IgG3 Cross-Adsorbed Secondary Antibody

Catalog No.	Quantity
PI31232	1 mg

Catalog No. PI31232 Supplier Invitrogen™ Supplier No. 31232

Description

Goat Polyclonal Secondary Antibody

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31232 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31232 reacts with the heavy chains of mouse IgG subclasses 1, 2a, 2b and 3, but not with the light chains of most mouse immunoglobulins. This antibody does not react against mouse IgM, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and rabbit serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Country of Origin: USA.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG1, IgG2a, IgG2b, IgG3 Cross-Adsorbed
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1.3 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with no preservative; pH 7.6
• Host Species: Goat
• Purification Method: Antigen affinity chromatography
• Quantity: 1 mg
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Mouse
• Content And Storage: 4°C
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: IgG

Frequently Asked Questions (FAQs)

I have two primary antibodies from the same host species that I wish to use to label the same cells. How do I choose secondary antibodies?

If the two primary antibodies are from different isotypes (such as mouse IgG1 and IgG2a), there are isotype-specific secondary antibodies to choose from. The other option is to make direct conjugates of the primary antibodies through the use of antibody label kits, reactive dyes, or custom conjugations.

Safety and Handling

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