Invitrogen™ Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, FITC

Goat Polyclonal Secondary Antibody

Supplier:  Invitrogen™ 31634

Catalog No. PI31634

Description

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31634 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31634 reacts with the heavy chains of mouse IgG2a, but not with heavy chains of other mouse IgG subclasses or with light chains of most mouse immunoglobulins. This antibody does not react against mouse IgM, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and rabbit serum proteins. However, these antibodies may cross-react with immunoglobulins from other species. Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: 7.6 μg/mg; ~ 3 moles FITC per mole IgG (lot-dependent). Reconstitute with 0.35 mL of distilled water. Country of Origin: USA.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG2a Cross-Adsorbed
• Classification: Polyclonal
• Conjugate: FITC
• Host Species: Goat
• Quantity: 500 μg
• Primary or Secondary: Secondary
• Content And Storage: 4°C, store in dark
• Form: Lyophilized

• Applications: Immunoprecipitation, Immunocytochemistry, Immunohistochemistry
• Concentration: 1.6 mg/mL
• Formulation: PBS with 15mg/mL BSA and 0.05% sodium azide; pH 7.6
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Product Type: Secondary Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov