Invitrogen™ Goat anti-Rabbit IgG Fc Superclonal™ Secondary Antibody, Alexa Fluor™ 488

Catalog No.	Quantity
50-003-32721	250 μL

Catalog No. 50-003-32721 Supplier Invitrogen™ Supplier No. SA510378AF488

Description

Goat Recombinant Superclonal Secondary Antibody

The Goat anti-Rabbit IgG Fc Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 488 is specific to Fc region of Rabbit IgG with moderate cross reactivity to Monkey IgG. It showed no cross-reactivity to any other immunoglobulins tested from other species. The sensitivity and specificity of each lot is confirmed using ELISA. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. Protein conjugates made with Alexa Fluor™ dyes produce fluorescence output that surpasses that of other spectrally similar fluorophore-labeled proteins. Alexa Fluor™ dyes are available in a wide selection of fluorescent colors and are more photostable than most other dyes. Photostability is a key characteristic of Alexa Fluor dye™ conjugates, allowing more time for image capture. In addition to their exceptional brightness and the wide selection of dyes across the spectrum, you'll get photostability you can rely on with Alexa Fluor™ dyes and dye conjugates. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Rabbit IgG Fc
• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Classification: Recombinant Superclonal
• Concentration: 1 mg/mL
• Conjugate: Alexa Fluor 488
• Formulation: Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.3-7.3
• Host Species: Goat
• Immunogen: Purified Rabbit IgG.
• Purification Method: Affinity chromatography
• Quantity: 250 μL
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Rabbit
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: IgG