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Goat Anti-Rabbit IgG (H&L) Antibody (HRP), ProSci
SDP

Catalog No. 16817891
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2 mg
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Catalog No. Quantity
16-817-891 2 mg
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Catalog No. 16-817-891 Supplier ProSci LLC Supplier No. XR99202000
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Description

Secondary Antibody

Goat Anti-Rabbit IgG (H&L) Antibody specifically detects Goat Anti-Mouse IgG (H&L) in Rabbit Samples. It is validated for ELISA, Western Blot, Immunohistochemistry.

Specifications

Antigen Goat Anti-Rabbit IgG (H&L)
Applications ELISA, Western Blot, Immunohistochemistry
Classification Polyclonal
Conjugate HRP
Dilution Peroxidase Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:20,000 - 1:100,000, Western Blot 1:2,000 - 1:10,000, Immunohistochemistry 1:1,000 - 1:5,000. (Optimal dilution should be determined by user).
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/mL Bovine Serum Albumin (BSA); 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Host Species Goat
Immunogen Rabbit IgG - whole molecule
Purification Method Peroxidase Conjugated Affinity Purified anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
Quantity 2 mg
Regulatory Status RUO
Primary or Secondary Secondary
Test Specificity Minimum Cross reactivity to Human Serum Proteins
Target Species Rabbit
Content And Storage Peroxidase Conjugated Affinity Purified anti-Rabbit IgG can be stored vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Form Lyophilized
Isotype IgG
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