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Invitrogen™ Goat anti-Rabbit IgG (H+L) Cross-Adsorbed ReadyProbes™ Secondary Antibody, Alexa Fluor™ 488

Catalog No. R37116
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Catalog No. R37116 Supplier Invitrogen™ Supplier No. R37116
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Description

Goat Polyclonal Secondary Antibody

No pipetting or dilutions required. Just tip and drip two drops per mL buffer to stain your cells.

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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Specifications

Antigen Rabbit IgG (H+L) Cross-Adsorbed
Applications Flow Cytometry, Western Blot, Immunocytochemistry
Classification Polyclonal
Conjugate Alexa Fluor 488
Formulation PBS with 0.1% BSA and <0.1% sodium azide
Host Species Goat
Immunogen Gamma Immunoglobin.
Purification Method Affinity chromatography
Quantity 2 x 2.5 mL
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Rabbit
Content And Storage 4°C, store in dark
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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Frequently Asked Questions (FAQs)

Can I use Goat anti-Rabbit IgG (H+L) Cross-Adsorbed ReadyProbes Secondary Antibody (Cat. No. R37116) on tissue sections (both paraffin-embedded or cryosections)?

Yes, Goat anti-Rabbit IgG (H+L) Cross-Adsorbed ReadyProbes Secondary Antibody (Cat. No. R37116) can be used on tissue sections (both paraffin-embedded or cryosections) but you may need a longer incubation time than indicated in the quick protocol. Two hours incubation at room temperature is the typical process, but we recommend optimizing incubation times with control samples.
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