Invitrogen™ Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, AP

Goat Polyclonal Secondary Antibody

Supplier:  Invitrogen™ G21079

Catalog No. G21079

Description

The specific activity of the AP conjugates is approximately 150-350 units/mg. One unit is defined as the amount of enzymatic activity required to hydrolyze 1.0 micromole of p nitrophenyl phosphate to p-nitrophenol and inorganic phosphate in 60 seconds at 21ºC, pH 10. Reconstituted using 0.5 mL of deionized water to make a 2 mg/mL stock solution.

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Rabbit IgG (H+L) Cross-Adsorbed
• Classification: Polyclonal
• Conjugate: Alkaline Phosphatase (AP)
• Host Species: Goat
• Purification Method: purified
• Regulatory Status: RUO
• Target Species: Rabbit
• Product Type: Secondary Antibody
• Isotype: IgG

• Applications: ELISA, Immunohistochemistry, Western Blot
• Concentration: 2 mg/mL
• Formulation: 50mM tris with 0.1M NaCl, 30mM trehalose and no preservative; pH 8.0
• Immunogen: Gamma Immunoglobins Heavy and Light chains.
• Quantity: 1 mg
• Primary or Secondary: Secondary
• Content And Storage: -20°C
• Form: Lyophilized