Invitrogen™ Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, PE

Catalog No.	Quantity
SA510384PE	500 μL

Catalog No. SA510384PE Supplier Invitrogen™ Supplier No. SA510384PE

Description

Goat Recombinant Superclonal Secondary Antibody

Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, PE detects both Fc and Fab2 fragment of Rabbit IgG. It has less than 5% cross reactivity to Mouse IgG. It showed no cross-reactivity to various species immunoglobulins tested. R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity, but not photostability. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Rabbit IgG (H+L)
• Applications: Flow Cytometry
• Classification: Recombinant Superclonal
• Concentration: 0.2 mg/mL
• Conjugate: PE
• Formulation: PBS with 5mM sodium azide; pH 7.4
• Host Species: Goat
• Immunogen: Purified Rabbit IgG.
• Purification Method: Affinity chromatography
• Quantity: 500 μL
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Rabbit
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: IgG