Invitrogen™ Goat anti-Rat IgG Fc Superclonal™ Secondary Antibody, PE

Catalog No.	Quantity
SA510388PE	500 μL

Catalog No. SA510388PE Supplier Invitrogen™ Supplier No. SA510388PE

Description

Goat Recombinant Superclonal Secondary Antibody

Goat anti-Rat IgG Fc Superclonal™ Recombinant Secondary Antibody, PE is specific to Fc fragment of Rat IgG. It can detect all subtypes of Rat IgG (Rat IgG1,2a,2b). It has less than 10% cross reactivity to Mouse IgG. It showed minimum to no cross-reactivity to various species immunoglobulins tested. R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity, but not photostability. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Rat IgG Fc
• Applications: Flow Cytometry
• Classification: Recombinant Superclonal
• Concentration: 0.2 mg/mL
• Conjugate: PE
• Formulation: PBS with 5mM sodium azide; pH 7.4
• Host Species: Goat
• Immunogen: Purified Rat IgG.
• Purification Method: Affinity chromatography
• Quantity: 500 μL
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Rat
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: IgG