Invitrogen™ Goat anti-Rat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ 555

Catalog No.	Quantity
50-011-30327	250 μL

Catalog No. 50-011-30327 Supplier Invitrogen™ Supplier No. SA510389AF555

Description

Goat Recombinant Superclonal Secondary Antibody

Goat anti-Rat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 555 detects both Fc and Fab2 fragment of Rat IgG. It can detect all subtypes of Rat IgG (Rat IgG1,2a,2b). It has very high reactivity to Hamster IgG and less than 20% cross reactivity to Mouse IgG. It showed no cross-reactivity to various species immunoglobulins tested. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. Protein conjugates made with Alexa Fluor™ dyes produce fluorescence output that surpasses that of other spectrally similar fluorophore-labeled proteins. Alexa Fluor™ dyes are available in a wide selection of fluorescent colors and are more photostable than most other dyes. Photostability is a key characteristic of Alexa Fluor dye™ conjugates, allowing more time for image capture. In addition to their exceptional brightness and the wide selection of dyes across the spectrum, you'll get photostability you can rely on with Alexa Fluor™ dyes and dye conjugates. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Rat IgG (H+L)
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Recombinant Superclonal
• Concentration: 1 mg/mL
• Conjugate: Alexa Fluor 555
• Formulation: Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.3-7.3
• Host Species: Goat
• Immunogen: Purified Rat IgG.
• Purification Method: Affinity chromatography
• Quantity: 250 μL
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Rat
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: IgG