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AdipoGen Grams Crystal Violet Solution
Supplier: AdipoGen CDXG0060L250
Chemical. CAS 548-62-9 main comp.. Formula . MW . Synthetic. Gram Crystal Violet Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Grams crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution Lugol’s solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange.
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