Invitrogen™ Human Granzyme A ELISA Kit

Catalog No.	Quantity
BMS2232	96 Tests

Catalog No. BMS2232 Supplier Invitrogen™ Supplier No. BMS2232

Description

ELISA

The Human Granzyme A ELISA quantitates Hu Granzyme A in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Granzyme A. Principle of the method The Human Granzyme A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells.

Specifications

• Accession Number: P12544
• Assay Range: 7.8-500 pg/mL
• Assay Sensitivity: 0.73 pg/mL
• Content And Storage: Store in refrigerator (2–8°C).
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Growth Factors & Receptors
• Sample Type: Cell Lysates, Plasma, Serum, Supernatant
• Substrate Type: Adaptor Proteins
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Autocrine thymic lymphoma granzyme-like serine protease; AW494114; BLT esterase; CTL tryptase; CTLA3; CTLA-3; Cytotoxic T-lymphocyte proteinase 1; Cytotoxic T-lymphocyte-associated serine esterase-3; fragmentin-1; granzyme 1; granzyme A; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease); granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); Granzyme-1; GZMA; H factor; Hanukah factor; Hanukah factor serine protease); Hanukkah factor; Hf; HFSP; Mtsp-1; SE1; serine esterase 1; t cell-specific serine protease 1; TSP1; TSP-1Autocrine thymic lymphoma granzyme-like serine protease; AW494114; BLT esterase; CTL tryptase; CTLA3; CTLA-3; Cytotoxic T-lymphocyte proteinase 1; Cytotoxic T-lymphocyte-associated serine esterase-3; fragmentin-1; granzyme 1; granzyme A; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease); granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); Granzyme-1; GZMA; H factor; Hanukah factor; Hanukah factor serine protease); Hanukkah factor; Hf; HFSP; Mtsp-1; SE1; serine esterase 1; t cell-specific serine protease 1; TSP1; TSP-1;
• Gene ID (Entrez): 3001
• Gene Symbol: GZMA
• Interassay CV: 8.5%
• Intraassay CV: 4.9%
• Kit Contents: Pre-coated 96 well plate, Standard (2 vials), Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard (2 vials), Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: CTL tryptase, Cytotoxic T-lymphocyte proteinase 1, Fragmentin-1, Granzyme-1, Hanukkah factor, H factor, HF
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: Granzyme A
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov