Invitrogen™ Human Granzyme A ELISA Kit

Description

ELISA

The Human Granzyme A ELISA quantitates Hu Granzyme A in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Granzyme A.

Principle of the method

The Human Granzyme A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells.

Specifications

• Accession Number: P12544
• Assay Range: 7.8 to 500 pg/mL
• Assay Sensitivity: 0.73 pg/ml
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3001
• Gene Symbol: GZMA
• Interassay CV: 8.5%
• Intraassay CV: 4.9
• Kit Contents: Pre-coated 96 well plate, Standard (2 vials), Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Granzyme A
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov