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Montana Molecular Green Cilia-targeted cADDis cAMP Assay - 10mL
Supplier: Montana Molecular D0201G010
Measure real-time cAMP signaling kinetics in the primary cilia with this live cell fluorescent assay. Add 5HT6-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Decreasing green fluorescence indicates ciliary cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cilia-targeted cAMP sensor BacMam and 500mM sodium butyrate in H2O.
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