Invitrogen™ Human GSK-3A, GST Tag Recombinant Protein

Catalog No.	Quantity
PV6126	10 μg

Catalog No. PV6126 Supplier Invitrogen™ Supplier No. PV6126

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. This recombinant fusion protein consists of full-length glycogen synthase kinase 3 alpha (GSK3A) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay. No special measures were taken to activate this kinase. Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay Expressed in insect cells GST tagged for use with Eu-labeled GST antibody Easily Screen for Kinase BindingThe LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors. The LanthatScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or tracer'...

Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638). Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.

Specifications

• Accession Number: P49840
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 2mM DTT, 0.02% NP-40, 150mM NaCl, 0.5mM EDTA, 50% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 2931
• Molecular Weight (g/mol): 78.4 kDa
• Name: Human GSK-3A, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 2700086H06Rik; DKFZp686D0638; FA; factor A; glycogen synthase kinase 3 alpha; glycogen synthase kinase 3 alpha b; glycogen synthase kinase 3 alpha b; glycogen synthase kinase-3 alpha; glycogen synthase kinase-3 alpha; GSK3; GSK-3 alpha; GSK-3[a]; GSK-3[b]; gsk3a; gsk3ab; gsk-3-alpha; OTTHUMP00000198368; Serine/threonine-protein kinase GSK3A
• Common Name: GSK3 alpha
• Gene Symbol: Gsk3a
• Kinase Family: GSK Kinase Family
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: Full length
• Content And Storage: Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% NP-40, 2 mM DTT and 50% glycerol. Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
• Assay Entry: Biochemical binding/kinetic, Biochemical phospho-specific activity (no antibody)
• Kinase Group: Ser⁄Thr Kinases: CMGC Group
• Readout: Kinetic
• Shipping Condition: Dry Ice
• System Compatibility: LanthaScreen™
• Tag Position: N-Terminal
• Target Entry: GSK3A
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Full Length
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases
• Research Category: Drug Discovery: Signal Transduction
• Product Line: LanthaScreen
• Purity or Quality Grade: 95% by SDS-PAGE