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Sigma Aldrich Fine Chemicals Biosciences GSTrapTM fast flow Cytiva
Supplier: Sigma Aldrich Fine Chemicals Biosciences GE17513001
GSTrap(TM) ff columns are prepacked Glutathione Sepharose(R) fast flow columns for fast convenient one-step purification of glutathione S-transferase (GST) tagged proteins.GST*-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap(TM) ff.GST-tagged proteins are eluted under mild nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium Glutathione Sepharose(R) 4 fast flow is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe peristaltic pump or liquid chromatography system such as AKTA design or fPLC(TM) System.* See the section f
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