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Description
- Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins
- This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins
- This can be used as the first step in refolding proteins or enzymes into their active form
- Urea and dithiothreitol (DTT) may also be necessary
- It is also used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties
- It is an inhibitor of RNase
Specifications
Specifications
| Melting Point | 175°C to 190°C |
| Color | White |
| Linear Formula | CH5N3·HCl |
| Quantity | 250 g |
| Solubility Information | Soluble in (6M aq soln): Relatively Clear to Cloudy Yellow Solution with Sediment on Standing. |
| Formula Weight | 95.53 |
| Grade | Practical |
| Physical Form | Powder |
| Chemical Name or Material | GUANIDINE HYDROCHLORIDE |
Safety and Handling
| Recommended Storage | Store at Room Temperature (15-30°C). Store Desiccated. |
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