Guanidine Hydrochloride, Ultra Pure grade, MP Biomedicals

Description

• Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins
• This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies
• This can be used as the first step in refolding proteins or enzymes into their active form
• Urea and dithiothreitol (DTT) may also be necessary
• Guanidine HCl is used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties
• It is an inhibitor of RNase
• Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins
• It apparently disrupts hydrogen bonds which hold the protein in its unique structure; however, there also is evidence suggesting that guanidine hydrocholoride may disrupt hydrophobic interactions by promoting the solubility of hydrophobic residues in aqueous solutions

Specifications

Chemical Identifiers

• CAS: 50-01-1
• Molecular Formula: CH6ClN3
• Molecular Weight (g/mol): 95.53
• MDL Number: MFCD00013026
• InChI Key: PJJJBBJSCAKJQF-UHFFFAOYSA-N
• Synonym: guanidine hydrochloride, guanidinium chloride, guanidine, monohydrochloride, aminoformamidine hydrochloride, aminomethanamidine hydrochloride, guanidine monohydrochloride, guanidine hcl, guanidine chloride, iminourea hydrochloride, carbamidine hydrochloride
• PubChem CID: 5742
• ChEBI: CHEBI:32735
• IUPAC Name: guanidine;hydrochloride
• SMILES: C(=N)(N)N.Cl

• Melting Point: 180°C to 190°C
• Color: White
• Linear Formula: CH5N3·HCl
• Quantity: 1 kg
• Solubility Information: Soluble in water (6 M - clear, colorless solution).
• Formula Weight: 95.53
• Percent Purity: ≥99%
• Grade: Ultra Pure
• Physical Form: Crystalline Powder
• Chemical Name or Material: GUANIDINE HYDROCHLORIDE

Safety and Handling

Recommended Storage

Store at Room Temperature (15-30°C). Store Desiccated.