Invitrogen™ Human MIP-1 delta/CCL15 ELISA Kit

Catalog No.	Quantity
EHCCL15X10	10 x 96 Tests

Catalog No. EHCCL15X10 Supplier Invitrogen™ Supplier No. EHCCL15X10

Description

ELISA

The Human Macrophage Inflammatory Protein-1 delta/CCL15 (MIP-1δ) ELISA quantitates Hu MIP-1δ in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MIP-1δ. Principle of the method The Human MIP-1δ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14).

Specifications

• Accession Number: Q16663
• Assay Range: 3-1000 pg/mL
• Assay Sensitivity: 3 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chemokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: C-C motif chemokine 15; C-C motif chemokine ligand 15; CCL15; CCL15(22-92); CCL15(25-92); CCL15(29-92); chemokine (C-C motif) ligand 15; chemokine CC-2; HCC-2; HCC-2Lkn-1; HMRP-2B; leukotactin 1; Leukotactin-1; LKN1; LKN-1; Macrophage inflammatory protein 5; MIP-1 delta; MIP-1D; MIP5; MIP-5; MIP-5MIP-1d; Mrp-2b; NCC3; NCC-3; NCC-3MIP-1D; NCC3MRP-2B; new CC chemokine 3; SCYA15; SCYA15member 15; SCYL3; small inducible cytokine subfamily A (Cys-Cys), member 15; small-inducible cytokine A15; SY15C-C motif chemokine 15; C-C motif chemokine ligand 15; CCL15; CCL15(22-92); CCL15(25-92); CCL15(29-92); chemokine (C-C motif) ligand 15; chemokine CC-2; HCC-2; HCC-2Lkn-1; HMRP-2B; leukotactin 1; Leukotactin-1; LKN1; LKN-1; Macrophage inflammatory protein 5; MIP-1 delta; MIP-1D; MIP5; MIP-5; MIP-5MIP-1d; Mrp-2b; NCC3; NCC-3; NCC-3MIP-1D; NCC3MRP-2B; new CC chemokine 3; SCYA15; SCYA15member 15; SCYL3; small inducible cytokine subfamily A (Cys-Cys), member 15; small-inducible cytokine A15; SY15; HCC-2; HMRP-2B; LKN-1; LKN1; MIP-1 delta; MIP-1D; MIP-5; MRP-2B; NCC-3; NCC3; SCYA15; SCYL3; SY15
• Gene ID (Entrez): 6359
• Gene Symbol: CCL15
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 3.3 μL, Serum 3.3 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: MIP-1 delta
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min