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CPC Scientific Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-psi-(COO)Ala-Ser-Lys(DABCYL)-NH2 (trifluoroacetate salt) 0.5MG
Supplier: CPC Scientific HCVP001A
A HCV protease substrate that incoporates an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm. As an internally quenced fluorogenic substrate, it can be used to monitor HCV NS3 protease activity.SEQUENCE: Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-psi-(COO)Ala-Ser-Lys(DABCYL)-NH2 (trifluoroacetate salt)ONE-LETTER SEQUENCE: Ac-DED(EDANS)-EE-Abu-psi-(COO)AS-K(DABCYL)-NH2MOLECULAR FORMULA: C68H89N15O25S1MOLECULAR WEIGHT: 1548.6STORAGE CONDITIONS: -20 5 CCAS REGISTRY NUMBER: SYNONYMS: Ac-
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