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Enzo Life Sciences CHEMILUM DE LYS HDAC/SIRT Chemiluminescent drug discovery kit (1x96wells)
Supplier: Enzo Life Sciences BMLAK5320001
The HDAC/SIRT Chemiluminescent Drug Discovery Kit is a complete assay system designed to measure histone deacetylase and sirtuin activity in cell or nuclear extracts, immunoprecipitates or purified enzymes. The HDAC Chemiluminescent Activity Assay is based on the unique CHEMILUM DE LYS Substrate and Developer combination. The CHEMILUM DE LYS system is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The assay procedure has three steps. First, the CHEMILUM DE LYS Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity . Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the CHEMILUM DE LYS Developer followed by Enhancer produces light. The reaction is luciferase-free Alternative name: Histone deacetylase fluorescent assay kit. Applications: Chemiluminescence, HTS Activity assay.
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