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Enzo Life Sciences FLUOR DE LYS HDAC3/NCOR1 fluorometric drug discovery kit (1x96wells)
Supplier: Enzo Life Sciences BMLAK5310001
A FLUOR DE LYS fluorescent assay system. The HDAC3/NCOR1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The assay procedure has two steps. First, the FLUOR DE LYS SIRT1 Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC3/NCOR1. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS Developer II produces a fluorophore. Alternative name: Histone deacetylase 3/Nuclear receptor corepressor 1 fluorometric drug discovery kit. Applications: Fluorescent detection, HTS Cell-based assays, Activity assay.
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