Gibco™ Fetal Bovine Serum, heat inactivated, qualified, Canada, One Shot™ format

Catalog No.	Quantity
A3840301	50 mL

Catalog No. A3840301 Supplier Gibco™ Supplier No. A3840301

Description

Heat-inactivated serum in a One-Shot 50-mL bottle increases workflow benefits, while maintaining serum quality. The 50-mL bottle eliminates the need to aliquot and reduces the risk of contamination.

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements.

• Serum category: Value (Standard)
  • For cell culture with robust cell lines
  • Good value for basic research
• Endotoxin level: ≤50 EU/mL (levels routinely ≤10 EU/mL)
• Hemoglobin level: ≤25 mg/dL
• Origin: countries meeting USDA importation requirements

Gibco sera: trusted quality for consistent performance

• ISO 13485-certified, processed in FDA-registered facilities
• Triple filtered at 0.1 μm

Heat-inactivated and convenient One-Shot 50-mL bottle format

Heat-inactivated serum in a One-Shot 50-mL bottle increases workflow benefits, while maintaining serum quality. The 50-mL bottle eliminates the need to aliquot and reduces the risk of contamination. Purchasing heat-inactivated serum eliminates the multiple steps necessary to heat inactivate and improves product integrity and consistency since the heat inactivation is done in a controlled environment.

One-Shot 50-mL bottle features include:

• Wide mouth, chamfered edge, and curved shape for easy pouring and pipetting
• Engineered to freeze/thaw without splitting or breaking
• Fits in test tube racks and frees up freezer space
• No labeling mishaps with our easy-to-read package label containing all of the information you need
• 33% less waste generated than aliquoting from 500-mL bottles

Specifications

• Content And Storage: Storage conditions: ≤-10°C
  Shipping conditions: Frozen
• Species: Bovine
• Age: Fetal
• Country of Origin: Canada
• Format: One Shot
• Product Type: Fetal Bovine Serum
• Quantity: 50 mL
• Serum Treatment: Heat-inactivated
• Sterility: Sterile
• Sterilization Method: Triple-filtered, 0.1 μm
• Purity or Quality Grade: Qualified
• Product Line: One Shot
• Shipping Condition: Frozen

Frequently Asked Questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

When should I use heat-inactivated serum?

Heat-inactivation of serum inactivates the “complements” in the serum. In general, it is not necessary to use heat-inactivated serum to culture most cell types. We recommend using heat-inactivated serum when working with immune type cells or for immunological applications.

I would like to order heat-inactivated FBS. What conditions do you use for heat-inactivation of FBS during the manufacturing process?

Serum is heat inactivated by heating at 56 degrees C in a water bath for 30 min, swirling the bottle approximately every 10 minutes.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.