Gibco™ HepaRG™ Cells, Cryopreserved

Catalog No.	Quantity
HPRGC10	1 vial

Catalog No. HPRGC10 Supplier Gibco™ Supplier No. HPRGC10

Description

HepaRG™ Cells, Cryopreserved

The HepaRG™ cell line is an immortalized hepatic cell line that retains many characteristics of primary human hepatocytes. HepaRG™ cells are terminally differentiated and provided in a convenient cryopreserved format. For scientists who need reproducible metabolism data, HepaRG™ cells are an in vitro tool that provides reproducible results in a metabolically complete and scalable system.

Obtain biologically relevant results from a metabolically complete system

Assess the drug-drug interaction potential of your compound

Experience reproducible results from a single population of cells

Biologically Relevant
HepaRG™ cells exhibit many characteristics of primary human hepatocytes including morphology, expression of key metabolic enzymes, expression of nuclear receptors, and drug transporters. Unlike HepG2 and Fa2N-4 cells, HepaRG™ cells have high P450 activity and complete expression of all nuclear receptors.

Predict Metabolism-based Drug-drug Interaction
HepaRG™ cells respond to prototypical P450 inducers and inhibitors to the same extent as primary hepatocytes, allowing HepaRG™ cells to be used to evaluate potential drug-drug interactions.

Infinitely Reproducible
HepaRG™ cells exhibit many characteristics of primary human hepatocytes and are essentially a single donor. These features allow users to obtain physiological relevant results for metabolism-based drug-drug interactions without the concern of donor variability and limited lot sizes that come with relying on donor tissue. (Note: The cells we provide are terminally differentiated).

HepaRG™ is a trademark of BioPredic International.

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Specifications

• Content And Storage: 1 vial, store in cryostorage dewar or -135°C freezer
• Age: Adult
• Cell Line: HepaRG
• Cell Type: Hepatocytes
• Form: Cryopreserved
• Gender: Female
• Species: Human
• Donor Source: Single Donor
• No. of Cells: 10 x 10⁶
• Product Type: Hepatocytes
• Quantity: 1 vial
• Shipping Condition: Dry Ice

Frequently Asked Questions (FAQs)

I have a sub-optimal monolayer confluency for my HepaRG cells. What should I do?

Here are possible causes and suggestions:

-Low seeding density: Follow recommended procedure for seeding the cells depending upon your application in the HepaRG Cell User Guide (http://www.thermofisher.com/us/en/home/references/protocols/drug-discovery/adme-tox-protocols/heparg-cell-user-guide.html).
-Low attachment efficiency; inadequate time allowed for attachment
--Low attachment efficiency may be due to:
---Poor-quality matrix: Use only Collagen I coated plates from recognized manufacturers.
---Cells not handled gently: Freezing and thawing procedures are stressful for cells and cause them to be fragile. Handle the cells gently.

I have an unexpected cell number for my HepaRG cells. Why is this?

Here are possible reasons:

-Improper thawing technique: Review Section 2.1: Protocol for thawing of HepaRG cells (https://www.thermofisher.com/us/en/home/references/protocols/drug-discovery/adme-tox-protocols/heparg-cell-user-guide.html#3).
-Thawing medium is not correct: Use the recommended medium - Review Section 1: Recommended materials, media, and cells (https://www.thermofisher.com/us/en/home/references/protocols/drug-discovery/adme-tox-protocols/heparg-cell-user-guide.html#2).
-Centrifugation speed is not correct: Follow the recommended speed for centrifugation. Review Section 2.1: Protocol for thawing of HepaRG cells (https://www.thermofisher.com/us/en/home/references/protocols/drug-discovery/adme-tox-protocols/heparg-cell-user-guide.html#3). Make sure that the centrifuge is calibrated. If the viability is very high but the cell number is low, then the centrifugation speed is too low - increase the speed and repeat centrifugation (this may require some optimization). By contrast, if the viability is lower than expected and the number of cells is very high, then the centrifugation speed is too high and is pelleting dead cells as well as viable cells.
-Loss of cells: After centrifugation, aspirate the supernatant while leaving a small volume on the pellet. Be careful not to aspirate the pellet.
-Improper counting technique: Review Section 2.2: Protocol for counting of HepaRG cells (https://www.thermofisher.com/us/en/home/references/protocols/drug-discovery/adme-tox-protocols/heparg-cell-user-guide.html#3). Ensure a homogeneous cell mixture prior to counting. Due to the normal presence of aggregates in the cell suspension, we recommend counting the cells with a hemocytometer.

My HepaRG cells have low post-thaw cell viability. Why is this?

Here are possible reasons:

-Storage temperature not maintained below -80 degrees C or repeated transient increase in temperature to RT
-Cells were thawed incorrectly
-Thawing medium is not correct or was at the incorrect temperature
-Cells were not handled gently
-Counting difficulty: Perform a repeat count with a new sample; avoid vortexing the cells to mix them
-Shipment temperature not maintained below -70 degrees C

After I use the HepaRG cells for an experiment, can I re-use them?

The cells are licensed for single-use applications.

How should the HepaRG cells be stored?

Upon receipt, cryovials must be stored in liquid or vapor phase nitrogen.

How are the HepaRG cells delivered?

The HepaRG cells are available as cryopreserved cells in vials. They are shipped in dry ice for an overnight delivery or in a vapor phase nitrogen container for longer shipments.

What are HepaRG cells?

HepaRG cells are a human hepatic progenitor cell line that retains many characteristics of primary human hepatocytes. HepaRG cells are terminally differentiated and provided in a convenient cryopreserved format. For scientists who need reproducible metabolism data, HepaRG cells are an in vitro tool that provides reproducible results in a metabolically complete and scalable system.

For Research Use Only. Not for use in diagnostic procedures.