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Cusabio Technology LLC Human hepatitis B virus e antigen (HBeAg) ELISA Kit
Supplier: Cusabio Technology LLC CSBE13557H
The Human hepatitis B virus e antigen (HBeAg) ELISA kit is used for qualitative identification of HBeAg in human serum and plasma. It employs the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with HBeAb. Samples or standards are pipetted into the wells with HRP-conjugated HBeAb. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of HBeAg bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm. It indicates the presence of HBeAg if the O.D. (optical density) of HBeAg is greater than or equal to the cutoff value (2.1× Average O.D. value of negative control). There is no HBeAg present in the sample if the O.D. is less than the cutoff value. One kit contains 96 wells.
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