Invitrogen™ Human HGF Instant ELISA™ Kit

Catalog No.	Quantity
50-246-657	128 Tests

Catalog No. 50-246-657 Supplier Invitrogen™ Supplier No. BMS2069INST

Description

ELISA

The Human Hepatocyte Growth Factor (Hu HGF) ELISA quantitates Hu HGF in human serum, plasma, cell culture supernatants, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu HGF. Principle of the method The Human HGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.

Specifications

• Accession Number: P14210
• Assay Range: 63-4000 pg/mL
• Assay Sensitivity: 5.5 pg/mL
• Content And Storage: Aluminum pouch(es) with a Microplate coated with antibody against human HGF, Biotin-Conjugate (anti-human HGF antibody), Streptavidin-HRP and Sample Diluent, lyophilized
  Aluminum pouch(es) with a human HGF Standard curve (coloured)
  1 bottle (25 mL) Wash Buffer Concentrate 20x(phosphate-buffered saline with 1% Tween 20)
  1 vial (15 mL) Substrate Solution (tetramethyl-benzidine)
  1 vial (12 mL) Sample Diluent (Use when an external predilution of the samples is needed)
  1 vial (15 mL) Stop Solution (1M Phosphoric acid)
  2 Adhesive Films
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: C230052L06Rik; DFNB39; Fibroblast derived tumor cytotoxic factor; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor; Hepatocyte growth factor (hepapoietin A; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain; Hepatopoeitin A; hepatopoeitin-A; HepatopoietinA; hepatopoietin-A; Hgf; HGF/SF; HGFB; HPTA; Lung fibroblast derived mitogen; lung fibroblast-derived mitogen; NK1; NK2; Scatter factor; scatter factor); SF; SF/HGFC230052L06Rik; DFNB39; Fibroblast derived tumor cytotoxic factor; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor; Hepatocyte growth factor (hepapoietin A; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain; Hepatopoeitin A; hepatopoeitin-A; HepatopoietinA; hepatopoietin-A; Hgf; HGF/SF; HGFB; HPTA; Lung fibroblast derived mitogen; lung fibroblast-derived mitogen; NK1; NK2; Scatter factor; scatter factor); SF; SF/HGF; DFNB39; F-TCFB; HPTA; SF; HGF
• Gene ID (Entrez): 3082
• Gene Symbol: Hgf
• Interassay CV: 8.3%
• Intraassay CV: 7.6%
• Kit Contents: Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Hepatopoietin-A, Scatter factor, SF
• Quantity: 128 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: -20°C
• Synonym: Hepatocyte Growth Factor, hepapoietin A, F-TCF
• Target: HGF
• Test Time: 15 min
• Total Assay Time: 3 hr 10 min

Safety and Handling

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