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Gibco™ Hibernate™-E Medium
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Catalog No. A1247601
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Description

Hibernate™-E Medium

Hibernate Medium is a CO2-independent nutrient medium for the maintenance of neural cells, tissue, and tissue slices. Hibernate-E Medium is optimized for embryonic cells or tissue in ambient carbon dioxide levels.

Hibernate Medium enables neurons to be maintained in ambient CO2 levels for up to 48 hours. This provides scientists with greater convenience and control while running bench-top live cell experiments, such as microscopy, flow cytometry, and other physiology studies. Hibernate Medium also allows for the preservation of viable brain tissue before processing for up to a month with refrigeration and maintenance of tissue slices.

Hibernate Medium may be combined with B-27 Supplements to yield a complete medium.

For other applications, we provide alternative formulations of Hibernate Medium and B-27 Supplements.

For established protocols using Hibernate Medium, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.
TRUSTED_SUSTAINABILITY

Specifications

Cell Type Neural Stem Cells
Classification Serum-free
Form Liquid
Product Type Stem Cell Media
Serum Level Serum-free
Sterility Sterile
With Additives Phenol Red
Product Line Hibernate
Quantity 500 mL
Shipping Condition Ambient
Tested For Cytotoxicity, Endotoxin, Sterility, pH, Osmolality
Content And Storage Store in refrigerator (2–8°C) and protect from light.
Culture Type Mammalian Cell Culture
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Frequently Asked Questions (FAQs)

Can the Hibernate-E Medium (Cat. No. A1247601) be used for plating and growing mouse primary hippocampal neurons?

Hibernate-E medium (Cat. No. A1247601) is designed for the maintenance of embryonic neural tissue and is suitable for short-term maintenance of cultured rat neurons at 37°C in ambient CO2 (up to 2 days) and long-term storage of viable brain tissue at 2 to 8°C (up to a month). However, it is not recommended for the initial plating and growing of neurons.

What medium can be used to maintain/manipulate neurons at ambient CO2?

We offer Gibco Hibernate Medium for this purpose. When supplemented with Gibco B-27 Supplement and Gibco GlutaMAX-1 Supplement, this medium can allow for the manipulation of neurons at ambient CO2 for at least 48 hours while retaining their viability, and preserve viable brain tissue for up to a month when stored at 4 degrees C. Two Hibernate media are offered:

- Gibco Hibernate-A Medium (Cat. No. A1247501) is formulated for postnatal neurons.
- Gibco Hibernate-E Medium (Cat. No. A1247601) is formulated for embryonic neurons.

These two media have the same formulation and differ only in osmolality.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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