Sigma Aldrich Fine Chemicals Biosciences High fidelity PCR Master

Supplier:  Sigma Aldrich Fine Chemicals Biosciences 12140314001

The 5-3 polymerase activity of Taq DNA Polymerase and the 3- 5 exonuclease of the proofreading polymerase combine for improved performance and results 3-5 exonuclease proofreading produces a threefold increase in DNA synthesis fidelity compared to Taq DNA polymerase.PCR products have both blunt ends and 3 single A-overhangs just like using Taq DNA polymerase ideal for blunt end cloning or T/A cloning.The High fidelity PCR Master is a ready-to-use double-concentrated master mix that contains all the reagents required to perform PCR (except template and primer). Water PCR Grade is also supplied.This powerful specific enzyme blend of the High fidelity PCR Master is designed to give PCR products with high yield high fidelity and high specificity from episomal and genomic DNA. The special enzyme blend is composed of two thermostable polymerases Taq DNA Polymerase and a thermostable polymerase with proofreading activity. Due to the inherent 3-5 exonuclease activity of the proofreadi

Catalog No. 50-290-6139